These guides cover a method or a subject as a whole: its principle, what it actually measures, what it allows you to conclude and what it does not. They are written for anyone who has to choose an approach, or to judge exactly what a result obtained by that approach is worth.
Each closes on the limits of the method and on the publications behind it. Most of these are work Inovarion contributed to.
For narrower, more practical questions — how to design a panel, how to prepare a sample — see the method notes.
Reading the transcriptome and the molecular layers
- scRNA-seq vs bulk RNA-seq: how to choose — what is gained and what is lost in moving from the average of a sample to the profile of each cell.
- Spatial transcriptomics: principle, limits and applications in neuroinflammation and myositis — measuring expression without erasing the geography of the tissue, and what resolution allows.
- Mapping chromatin: from ChIP-seq to CUT&Tag — locating a mark on the genome, and the shift under way toward guided nucleases.
- Metabolomics — the layer closest to the phenotype, and the identification bottleneck.
- Multi-omics integration — what disagreements between layers reveal, and how to tell a discovery from an artifact.
Characterizing a biological context
- Characterizing the tumor immune microenvironment — why the spatial organization of the infiltrate carries information that abundance alone does not.
- B cell memory and vaccine response: what single-cell adds — what the quality of memory says about protection, beyond antibody titer.
- Digital pathology and quantitative image analysis — turning a visual appraisal into a reproducible measurement, and what that changes around a threshold.
Modeling, perturbing, building
- Humanized mouse models and in vivo preclinical models: an overview — which model answers which question, and at the price of which simplification.
- iPSC-derived cell models: principle, contributions and limits — human material carrying the patient’s mutation, and the value of the isogenic control.
- CRISPR-Cas9 screens: principles, strategies and applications — interrogating thousands of genes by perturbation, and what a screen hit really establishes.
- Synthetic biology — building the object that makes the question measurable, and why modularity is not a given.
Designing a candidate
- Single-domain antibodies (VHH) in hemostasis: research, diagnosis, therapy — what small size makes possible, from research tool to therapeutic candidate.
updated August 2026